fig3

Figure 3. Activation of apoptosis in the Myh6-Mcm^Tam:Dsp^F/F mice. (A) GSEA plot predicting activation of the TNFα signaling pathway in the Myh6-Mcm^Tam:Dsp^F/F myocytes. (B) GSEA plot showing enrichment of genes involved in apoptosis in the Myh6-Mcm^Tam:Dsp^F/F myocytes. (C) Heat map of the DEGs showing upregulation of most of the genes involved in TNFα signaling pathway in the Myh6-Mcm^Tam:Dsp^F/F myocytes. (D) Heat map of the DEGs showing upregulation of most of the genes involved in the apoptosis in the Myh6-Mcm^Tam:Dsp^F/F myocytes. (E) Thin myocardial sections stained with the TUNEL assay. (F) Quantitative data showing the percent of nuclei stained positive for the TUNEL assay in the experimental groups. (G) Dot plots showing transcript levels of selected markers of apoptosis, as detected by RT-PCR of cardiac myocyte RNA extracts. (H) Immunoblots showing expression of CASP8 and CASP3 along with that of VCL, the latter as a control for the loading condition. (I) Quantitative data representing the blots shown in panel H. (J) Immunoblots showing expression of BCL2 and BAD along with that of VCL, the latter as a control for the loading condition. (K) Quantitative data representing the blots shown in panel J.