fig2

Figure 2. Application of proteomic analysis for amyloidosis typing. (top) Mayo Clinic technique from formalin-fixed and paraffin-embedded (FFPE) specimens. After Congo red (CR) staining, amyloid deposits are cut from tissue slices and undergo laser microdissection (LMD) with a fluorescence module leading to a strong enhancement of deposits. The material is then suitable for liquid chromatography and tandem mass spectrometry analysis followed by bioinformatic analysis. (bottom) Shotgun liquid chromatography and tandem mass spectrometry analysis. Semiquantitative label-free simultaneous comparison of the amyloid positive samples from both fresh fat and FFPE against an average map of negative control tissue. Amyloid identification is based on the estimation of the alpha value, representing the normalized relative abundance of each known amyloid protein compared to controls. Modified from^[42].