fig3

Figure 3. Like mutations in known CCM3-GCKIII signaling pathway components, furry and Mo25 loss of function result in tube dilations in tracheal terminal cells. (A) The most proximal part of a wild type control terminal cell clone (GFP positive labeling of homozygous cells) is shown, depicting the portion of the terminal cell that extends from its intercellular junction with a neighboring stalk cell, at the bottom of the image, to the terminal cell nucleus, at the top of image. In wild type cells the lumen tapers very gradually and evenly from proximal (bottom) to distal (top). (B) In wheezy/GckIII mutant terminal cells, prominent transition zone (between intercellular junction and terminal cell nucleus) dilations are detected (<). Terminal cells mutant or knocked down for tao, ccm3, trc (not shown, see^[20,21]), (C) Mo25, or (D) furry show identical transition zone dilations. Scale bar = 10 microns.