fig2

Figure 2. Like mutations in furry, loss of CCM3 or GCKIII function cause wing hair number and polarity defects. (A) Anterior wing margin of a control wild type (OR-R) fly is shown. Note that individual wing hairs are aligned, and point towards the distal wing tip (to the right). In flies mosaic for furry (border between cells in the mutant clone and wild type cells outlined in red) (B), or expressing a dominant negative (non-phophorylatable) GCKIII isoform (GCKIII_T167A) (C), orientation of the wing hairs is disrupted and (red circles) two or more wing hairs per cell may be produced. (D) Wild type wing hairs in the area immediately proximal to the crossvein connecting veins 3 and 4 (dark pigmentation running vertically on the left of image) are shown. Note the presence of single wing hairs per cell that is oriented towards the distal wing tip (right). In furry clones (E) (clone borders marked in red), or wing cells expressing a dominant negative (non-phophorylatable) GCKIII isoform (F, GCKIII_T167A), orientation of the wing hairs is disrupted and (red circles) two or more wing hairs per cell are produced. (G) In wings that are mosaic for loss of ccm3 function (clone outlined in red), mutant cells produce multiple wing hairs and display altered orientation. We note that in addition to the number of wing hairs per cell, mutations in the CCM3/GCKIII-Trc/Fry pathway may also affect planar cell polarity so that the orientation of the hairs appear perturbed. Scale bar in G (for A-G) = 10 microns.