fig2

Figure 2. Targeted deletion of Aoc3 gene in mouse abolishes VAP-1/SSAO oxidative activity without notable compensation of MAO activities. SSAO-dependent oxidation of [¹⁴C]-benzylamine (top) or MAO-dependent oxidation of [¹⁴C]-tyramine (bottom) is represented for various organs from WT (closed columns) and AOC3KO (open columns) mice. Each column is mean ± SEM of eight WT and AOC3KO mice. For the SSAO activity (top), the difference between the two genotypes is at P < 0.001, irrespective of the tissue, while there is no genotype effect on MAO activity (bottom). Enzymatic oxidase activity of tissue homogenates is normalized per mg of DNA since protein content was highly different among the tested organs. INWAT: Intra-abdominal white adipose tissues; SCWAT: subcutaneous white adipose tissues.